[HTML][HTML] Glucose-induced cytosolic pH changes in β-cells and insulin secretion are not causally related: studies in islets lacking the Na+/H+ exchanger NHE1
The contribution of Na+/H+ exchange (achieved by NHE proteins) to the regulation of β-cell
cytosolic pH c, and the role of pH c changes in glucose-induced insulin secretion are
disputed and were examined here. Using real-time PCR, we identified plasmalemmal NHE1
and intracellular NHE7 as the two most abundant NHE isoforms in mouse islets. We,
therefore, compared insulin secretion, cytosolic free Ca 2+([Ca 2+] c) and pH c in islets from
normal mice and mice bearing an inactivating mutation of NHE1 (Slc9A1-swe/swe). The …
cytosolic pH c, and the role of pH c changes in glucose-induced insulin secretion are
disputed and were examined here. Using real-time PCR, we identified plasmalemmal NHE1
and intracellular NHE7 as the two most abundant NHE isoforms in mouse islets. We,
therefore, compared insulin secretion, cytosolic free Ca 2+([Ca 2+] c) and pH c in islets from
normal mice and mice bearing an inactivating mutation of NHE1 (Slc9A1-swe/swe). The …
