Critical analytical evaluation of promising markers for sarcopenia
We tested and validated irisin (IRI), myostatin (MYO), PIIINP, osteoglycin (OGN), TMEM119
(TMEM) and activin A (AA) and established the analytical performance, reference range and
stability (considered unstable if more than 20% increase/decrease in the levels was
observed in more than 10% of the samples). We were unable to obtain a valuable
calibration curve with the Cusabio kits (TMEME and OGN). Coefficient of variation (CV) was
too high for IRI (CV 17–30%), but were≤ 10% for the 3 other analytes. AA and MYO were …
(TMEM) and activin A (AA) and established the analytical performance, reference range and
stability (considered unstable if more than 20% increase/decrease in the levels was
observed in more than 10% of the samples). We were unable to obtain a valuable
calibration curve with the Cusabio kits (TMEME and OGN). Coefficient of variation (CV) was
too high for IRI (CV 17–30%), but were≤ 10% for the 3 other analytes. AA and MYO were …