[HTML][HTML] Validation of a next-generation sequencing–based protocol for 24-chromosome aneuploidy screening of blastocysts
Objective To validate a 24-chromosome aneuploidy preimplantation genetic screening
protocol based on multiple annealing and looping-based amplification cycle (MALBAC) and
next-generation sequencing (NGS). Design Single-nucleotide polymorphism (SNP) array
and MALBAC-NGS analysis. Setting University-affiliated in vitro fertilization (IVF) center.
Patient (s) Fifteen women from whom 30 blastocysts were obtained for genotyping.
Intervention (s) Not applicable. Main Outcome Measure (s) Chromosomal status comparison …
protocol based on multiple annealing and looping-based amplification cycle (MALBAC) and
next-generation sequencing (NGS). Design Single-nucleotide polymorphism (SNP) array
and MALBAC-NGS analysis. Setting University-affiliated in vitro fertilization (IVF) center.
Patient (s) Fifteen women from whom 30 blastocysts were obtained for genotyping.
Intervention (s) Not applicable. Main Outcome Measure (s) Chromosomal status comparison …