[PDF][PDF] Assessing computational amino acid β-turn propensities with a phage-displayed combinatorial library and directed evolution
Structure propensities of amino acids are important determinants in guiding proteins' local
and global structure formation. We constructed a phage display library—a hexa-HIS tag
upstream of a CXXC (X stands for any of the 20 natural amino acids) motif appending N-
terminal to the minor capsid protein pIII of M13KE filamentous phage—and developed a
novel directed-evolution procedure to select for amino acid sequences forming increasingly
stable β-turns in the disulfide-bridged CXXC motif. The sequences that emerged from the …
and global structure formation. We constructed a phage display library—a hexa-HIS tag
upstream of a CXXC (X stands for any of the 20 natural amino acids) motif appending N-
terminal to the minor capsid protein pIII of M13KE filamentous phage—and developed a
novel directed-evolution procedure to select for amino acid sequences forming increasingly
stable β-turns in the disulfide-bridged CXXC motif. The sequences that emerged from the …
Summary
Structure propensities of amino acids are important determinants in guiding proteins' local and global structure formation. We constructed a phage display library—a hexa-HIS tag upstream of a CXXC (X stands for any of the 20 natural amino acids) motif appending N-terminal to the minor capsid protein pIII of M13KE filamentous phage—and developed a novel directed-evolution procedure to select for amino acid sequences forming increasingly stable β-turns in the disulfide-bridged CXXC motif. The sequences that emerged from the directed-evolution cycles were in good agreement with type II β-turn propensities derived from surveys of known protein structures, in particular, Pro-Gly forming a type II β-turn. The agreement strongly supported the notion that β-turn formation plays an active role in initiating local structure folding in proteins.
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