[HTML][HTML] Consumption of a high energy density diet triggers microbiota dysbiosis, hepatic lipidosis, and microglia activation in the nucleus of the solitary tract in rats
Nutrition & Diabetes, 2020•nature.com
Introduction Obesity is a multifactorial chronic inflammatory disease. Consumption of high
energy density (HED) diets is associated with hyperphagia, increased body weight and body
fat accumulation, and obesity. Our lab has previously shown that short-term (4 weeks)
consumption of a HED diet triggers gut microbiota dysbiosis, gut inflammation, and
reorganization of the gut-brain vagal communication. Objetives The aim of this study was to
investigate the effect of long-term (6 months) consumption of HED diet on body composition …
energy density (HED) diets is associated with hyperphagia, increased body weight and body
fat accumulation, and obesity. Our lab has previously shown that short-term (4 weeks)
consumption of a HED diet triggers gut microbiota dysbiosis, gut inflammation, and
reorganization of the gut-brain vagal communication. Objetives The aim of this study was to
investigate the effect of long-term (6 months) consumption of HED diet on body composition …
Introduction
Obesity is a multifactorial chronic inflammatory disease. Consumption of high energy density (HED) diets is associated with hyperphagia, increased body weight and body fat accumulation, and obesity. Our lab has previously shown that short-term (4 weeks) consumption of a HED diet triggers gut microbiota dysbiosis, gut inflammation, and reorganization of the gut-brain vagal communication.
Objetives
The aim of this study was to investigate the effect of long-term (6 months) consumption of HED diet on body composition, gut microbiome, hepatocellular lipidosis, microglia activation in the nucleus of the solitary tract, and systemic inflammation.
Methods
Male Sprague–Dawley rats were fed a low energy density (LED) diet for 2 weeks and then switched to a HED diet for 26 weeks. Twenty-four-hour food intake, body weight, and body composition were measured twice a week. Blood serum and fecal samples were collected at baseline, 1, 4, 8, and 26 weeks after introduction of the HED diet. Serum samples were used to measure insulin, leptin, and inflammatory cytokines using Enzyme-linked Immunosorbent Assay. Fecal samples were assessed for 16 S rRNA genome sequencing.
Results
HED diet induced microbiota dysbiosis within a week of introducing the diet. In addition, there was significant microglia activation in the intermediate NTS and marked hepatic lipidosis after 4 weeks of HED diet. We further observed changes in the serum cytokine profile after 26 weeks of HED feeding.
Conclusions
These data suggest that microbiota dysbiosis is the first response of the organism to HED diets, followed by increased liver fat accumulation, microglia activation in the brain, and circulating levels of inflammatory markers. To our knowledge, this is the first study to present longitudinal and cross-sectional results on effect of long-term consumption of HED diets on all these parameters in a single cohort of animals.
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