[HTML][HTML] Binding of filamin to the C-terminal tail of the calcitonin receptor controls recycling
T Seck, R Baron, WC Horne - Journal of Biological Chemistry, 2003 - ASBMB
Many G protein-coupled receptors undergo endocytosis, but the mechanisms involved in
endocytic sorting and recycling remain to be fully elucidated. We found that the G protein-
coupled calcitonin receptor (CTR) undergoes tonic internalization and accumulates within
the cell. Using a fluorescence loss in photobleaching assay, we classified these vesicles
functionally as recycling vesicles. In a two-hybrid screening, we found that the actin-binding
protein filamin interacted with the C-terminal tail of the CTR. The degradation of the receptor …
endocytic sorting and recycling remain to be fully elucidated. We found that the G protein-
coupled calcitonin receptor (CTR) undergoes tonic internalization and accumulates within
the cell. Using a fluorescence loss in photobleaching assay, we classified these vesicles
functionally as recycling vesicles. In a two-hybrid screening, we found that the actin-binding
protein filamin interacted with the C-terminal tail of the CTR. The degradation of the receptor …