[PDF][PDF] Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes
F Crawford, H Kozono, J White, P Marrack, J Kappler - Immunity, 1998 - cell.com
F Crawford, H Kozono, J White, P Marrack, J Kappler
Immunity, 1998•cell.comMultimeric soluble MHC class II molecules stably occupied with covalently attached peptides
bind with appropriate specificity to T cell hybridomas and T cells from T cell receptor
transgenic mice. There is a direct correlation between soluble T cell receptor affinity for
monomeric MHC/peptide and level of binding of multimeric MHC/peptide to T cells. While
binding of the multimeric MHC/peptide complex is proportional to T cell receptor affinity and
expression level, there is little influence of T cell CD4.
bind with appropriate specificity to T cell hybridomas and T cells from T cell receptor
transgenic mice. There is a direct correlation between soluble T cell receptor affinity for
monomeric MHC/peptide and level of binding of multimeric MHC/peptide to T cells. While
binding of the multimeric MHC/peptide complex is proportional to T cell receptor affinity and
expression level, there is little influence of T cell CD4.
Abstract
Multimeric soluble MHC class II molecules stably occupied with covalently attached peptides bind with appropriate specificity to T cell hybridomas and T cells from T cell receptor transgenic mice. There is a direct correlation between soluble T cell receptor affinity for monomeric MHC/peptide and level of binding of multimeric MHC/peptide to T cells. While binding of the multimeric MHC/peptide complex is proportional to T cell receptor affinity and expression level, there is little influence of T cell CD4.
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