[HTML][HTML] Origin of Enriched Regulatory T Cells in Patients Receiving Combined Kidney–Bone Marrow Transplantation to Induce Transplantation Tolerance
B Sprangers, S DeWolf, TM Savage, T Morokata… - American Journal of …, 2017 - Elsevier
We examined tolerance mechanisms in patients receiving HLA-mismatched combined
kidney–bone marrow transplantation (CKBMT) that led to transient chimerism under a
previously published nonmyeloablative conditioning regimen (Immune Tolerance Network
study 036). Polychromatic flow cytometry and high-throughput sequencing of T cell receptor-
β hypervariable regions of DNA from peripheral blood regulatory T cells (Tregs) and CD4
non-Tregs revealed marked early enrichment of Tregs (CD3+ CD4+ CD25 high CD127 low …
kidney–bone marrow transplantation (CKBMT) that led to transient chimerism under a
previously published nonmyeloablative conditioning regimen (Immune Tolerance Network
study 036). Polychromatic flow cytometry and high-throughput sequencing of T cell receptor-
β hypervariable regions of DNA from peripheral blood regulatory T cells (Tregs) and CD4
non-Tregs revealed marked early enrichment of Tregs (CD3+ CD4+ CD25 high CD127 low …