Analysis of very long-chain fatty acids using electrospray ionization mass spectrometry
F Valianpour, JJM Selhorst, LEM van Lint… - Molecular genetics and …, 2003 - Elsevier
Elevated levels of very long-chain fatty acids (VLCFA) in plasma and tissues are the
biochemical hallmark for patients with X-linked adrenoleukodystrophy (X-ALD). Current
methods for the determination of VLCFA levels are laborious and time-consuming. We
describe a rapid and easy method using electrospray ionization mass spectrometry (ESI-
MS) with deuterated internal standards. VLCFA are hydrolyzed, extracted, and quantified in
less than 4h. This includes 2h of hydrolysis and 4min of quantification. We validated the …
biochemical hallmark for patients with X-linked adrenoleukodystrophy (X-ALD). Current
methods for the determination of VLCFA levels are laborious and time-consuming. We
describe a rapid and easy method using electrospray ionization mass spectrometry (ESI-
MS) with deuterated internal standards. VLCFA are hydrolyzed, extracted, and quantified in
less than 4h. This includes 2h of hydrolysis and 4min of quantification. We validated the …