[HTML][HTML] Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils
MA Gijón, WR Riekhof, S Zarini, RC Murphy… - Journal of Biological …, 2008 - ASBMB
The cycle of deacylation and reacylation of phospholipids plays a critical role in regulating
availability of arachidonic acid for eicosanoid production. The major yeast lysophospholipid
acyltransferase, Ale1p, is related to mammalian membrane-bound O-acyltransferase
(MBOAT) proteins. We expressed four human MBOATs in yeast strains lacking Ale1p and
studied their acyl-CoA and lysophospholipid specificities using novel mass spectrometry-
based enzyme assays. MBOAT1 is a lysophosphatidylserine (lyso-PS) acyltransferase with …
availability of arachidonic acid for eicosanoid production. The major yeast lysophospholipid
acyltransferase, Ale1p, is related to mammalian membrane-bound O-acyltransferase
(MBOAT) proteins. We expressed four human MBOATs in yeast strains lacking Ale1p and
studied their acyl-CoA and lysophospholipid specificities using novel mass spectrometry-
based enzyme assays. MBOAT1 is a lysophosphatidylserine (lyso-PS) acyltransferase with …