[HTML][HTML] Measurement of lysophospholipid acyltransferase activities using substrate competition [S]
SA Martin, MA Gijón, DR Voelker, RC Murphy - Journal of lipid research, 2014 - ASBMB
Lysophospholipid acyltransferases (LPATs) incorporate fatty acyl chains into phospholipids
via a CoA-dependent mechanism and are important in remodeling phospholipids to
generate the molecular species of phospholipids found in cells. These enzymes use one
lysophospholipid and one acyl-CoA ester as substrates. Traditional enzyme activity assays
engage a single substrate pair, whereas in vivo multiple molecular species exist. We
describe here an alternative biochemical assay that provides a mixture of substrates …
via a CoA-dependent mechanism and are important in remodeling phospholipids to
generate the molecular species of phospholipids found in cells. These enzymes use one
lysophospholipid and one acyl-CoA ester as substrates. Traditional enzyme activity assays
engage a single substrate pair, whereas in vivo multiple molecular species exist. We
describe here an alternative biochemical assay that provides a mixture of substrates …