[HTML][HTML] Ceramide generation is sufficient to account for the inhibition of the insulin-stimulated PKB pathway in C2C12 skeletal muscle cells pretreated with palmitate
C Schmitz-Peiffer, DL Craig, TJ Biden - Journal of Biological Chemistry, 1999 - ASBMB
We have employed C2C12 myotubes to investigate lipid inhibition of insulin-stimulated
signal transduction and glucose metabolism. Cells were preincubated for 18 h in the
absence or presence of free fatty acids (FFAs) and stimulated with insulin, and the effects on
glycogen synthesis and signaling intermediates were determined. While the unsaturated
FFAs oleate and linoleate inhibited both basal and insulin-stimulated glycogen synthesis,
the saturated FFA palmitate reduced only insulin-stimulated glycogen synthesis, and was …
signal transduction and glucose metabolism. Cells were preincubated for 18 h in the
absence or presence of free fatty acids (FFAs) and stimulated with insulin, and the effects on
glycogen synthesis and signaling intermediates were determined. While the unsaturated
FFAs oleate and linoleate inhibited both basal and insulin-stimulated glycogen synthesis,
the saturated FFA palmitate reduced only insulin-stimulated glycogen synthesis, and was …