Transient cold shock enhances zinc-finger nuclease–mediated gene disruption
Nature methods, 2010•nature.com
Zinc-finger nucleases (ZFNs) are powerful tools for editing the genomes of cell lines and
model organisms. Given the breadth of their potential application, simple methods that
increase ZFN activity, thus ensuring genome modification, are highly attractive. Here we
show that transient hypothermia generally and robustly increased the level of stable, ZFN-
induced gene disruption, thereby providing a simple technique to enhance the experimental
efficacy of ZFNs.
model organisms. Given the breadth of their potential application, simple methods that
increase ZFN activity, thus ensuring genome modification, are highly attractive. Here we
show that transient hypothermia generally and robustly increased the level of stable, ZFN-
induced gene disruption, thereby providing a simple technique to enhance the experimental
efficacy of ZFNs.
Abstract
Zinc-finger nucleases (ZFNs) are powerful tools for editing the genomes of cell lines and model organisms. Given the breadth of their potential application, simple methods that increase ZFN activity, thus ensuring genome modification, are highly attractive. Here we show that transient hypothermia generally and robustly increased the level of stable, ZFN-induced gene disruption, thereby providing a simple technique to enhance the experimental efficacy of ZFNs.
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