Complement receptor C5aR1/CD88 and dipeptidyl peptidase-4/CD26 define distinct hematopoietic lineages of dendritic cells

H Nakano, TP Moran, K Nakano, KE Gerrish… - The Journal of …, 2015 - journals.aai.org
H Nakano, TP Moran, K Nakano, KE Gerrish, CD Bortner, DN Cook
The Journal of Immunology, 2015journals.aai.org
Differential display of the integrins CD103 and CD11b are widely used to distinguish two
major dendritic cell (DC) subsets in nonlymphoid tissues. CD103+ DCs arise from FLT3-
dependent DC precursors (preDCs), whereas CD11b hi DCs can arise either from preDCs
or FLT3-independent monocytes. Functional characterization of these two lineages of
CD11b hi DCs has been hindered by the lack of a widely applicable method to distinguish
between them. We performed gene expression analysis of fractionated lung DCs from …
Abstract
Differential display of the integrins CD103 and CD11b are widely used to distinguish two major dendritic cell (DC) subsets in nonlymphoid tissues. CD103+ DCs arise from FLT3-dependent DC precursors (preDCs), whereas CD11b hi DCs can arise either from preDCs or FLT3-independent monocytes. Functional characterization of these two lineages of CD11b hi DCs has been hindered by the lack of a widely applicable method to distinguish between them. We performed gene expression analysis of fractionated lung DCs from C57BL/6 mice and found that monocyte-derived DCs (moDCs), including CD11b hi Ly-6C lo tissue-resident and CD11b hi Ly-6C hi inflammatory moDCs, express the complement 5a receptor 1/CD88, whereas preDC-derived conventional DCs (cDCs), including CD103+ and CD11b hi cDCs, express dipeptidyl peptidase-4/CD26. Flow cytometric analysis of multiple organs, including the kidney, liver, lung, lymph nodes, small intestine, and spleen, confirmed that reciprocal display of CD88 and CD26 can reliably distinguish FLT3-independent moDCs from FLT3-dependent cDCs in C57BL/6 mice. Similar results were obtained when DCs from BALB/c mice were analyzed. Using this novel approach to study DCs in mediastinal lymph nodes, we observed that most blood-derived lymph node–resident DCs, as well as tissue-derived migratory DCs, are cDCs. Furthermore, cDCs, but not moDCs, stimulated naive T cell proliferation. We anticipate that the use of Abs against CD88 and CD26 to distinguish moDCs and cDCs in multiple organs and mouse strains will facilitate studies aimed at assigning specific functions to distinct DC lineages in immune responses.
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