Comparative analysis of the efficiency and specificity of myeloid-Cre deleting strains using ROSA-EYFP reporter mice

CL Abram, GL Roberge, Y Hu, CA Lowell - Journal of immunological …, 2014 - Elsevier
Since the first example of conditional gene targeting in mice in 1994, the use of Cre
recombinase and loxP flanked sequences has become an invaluable technique to generate
tissue and temporal specific gene knockouts. The number of mouse strains expressing
floxed-gene sequences, and tissue-specific or temporal-specific Cre-recombinase that have
been reported in the literature has grown exponentially. However, increased use of this
technology has highlighted several problems that can impact the interpretation of any …