[HTML][HTML] Pulse-chase SILAC–based analyses reveal selective oversynthesis and rapid turnover of mitochondrial protein components of respiratory complexes
DF Bogenhagen, JD Haley - Journal of Biological Chemistry, 2020 - ASBMB
Mammalian mitochondria assemble four complexes of the respiratory chain (RCI, RCIII,
RCIV, and RCV) by combining 13 polypeptides synthesized within mitochondria on
mitochondrial ribosomes (mitoribosomes) with over 70 polypeptides encoded in nuclear
DNA, translated on cytoplasmic ribosomes, and imported into mitochondria. We have
previously observed that mitoribosome assembly is inefficient because some mitoribosomal
proteins are produced in excess, but whether this is the case for other mitochondrial …
RCIV, and RCV) by combining 13 polypeptides synthesized within mitochondria on
mitochondrial ribosomes (mitoribosomes) with over 70 polypeptides encoded in nuclear
DNA, translated on cytoplasmic ribosomes, and imported into mitochondria. We have
previously observed that mitoribosome assembly is inefficient because some mitoribosomal
proteins are produced in excess, but whether this is the case for other mitochondrial …