Common reabsorption system of 1, 5-anhydro-D-glucitol, fructose, and mannose in rat renal tubule

T Yamanouchi, T Shinohara, N Ogata… - … et Biophysica Acta (BBA …, 1996 - Elsevier
T Yamanouchi, T Shinohara, N Ogata, Y Tachibana, I Akaoka, H Miyashita
Biochimica et Biophysica Acta (BBA)-General Subjects, 1996Elsevier
1, 5-Anhydro-d-glucitol (AG) is a major polyol, 99.9% of which is reabsorbed by the kidney.
However, such reabsorption is inhibited by competition with glucose excreted in excess, ie,
glucosuria. Under such conditions, AG is excreted into the urine. We administered various
types of sugars to rats by continuous intravenous infusion for two hours to evaluate the
competition between AG and these sugars for renal reabsorption in vivo. The reabsorption of
AG was significantly inhibited by competition with fructose and mannose. The excretion of …
1,5-Anhydro-d-glucitol (AG) is a major polyol, 99.9% of which is reabsorbed by the kidney. However, such reabsorption is inhibited by competition with glucose excreted in excess, i.e., glucosuria. Under such conditions, AG is excreted into the urine. We administered various types of sugars to rats by continuous intravenous infusion for two hours to evaluate the competition between AG and these sugars for renal reabsorption in vivo. The reabsorption of AG was significantly inhibited by competition with fructose and mannose. The excretion of AG in the 120 min after a load of 3.64 mmol of fructose was 1.99 ± 0.33 μmol, that after 3.64 mmol of mannose loading was 2.34 ± 0.43 μmol. These levels were comparable to the AG excretion observed after the administration of the same amount of glucose (3.87 ± 0.61 μmol). No competition was observed with sucrose, xylose, myoinositol or galactose. The reabsorption of fructose and mannose was significantly inhibited by the presence of AG (P < 0.001) after a mixed load. Results suggest that AG is reabsorbed in the renal tubule by an AG/fructose/mannose-common transport system that is distinct from the major glucose reabsorption system. These findings may help to clarify the specific transport systems for various sugars in the renal tubule, as well as their physiological importance.
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