[HTML][HTML] The Metabolic Origins of Mannose in Glycoproteins*♦
M Ichikawa, DA Scott, ME Losfeld, HH Freeze - Journal of Biological …, 2014 - ASBMB
Mannose in N-glycans is derived from glucose through phosphomannose isomerase (MPI,
Fru-6-P↔ Man-6-P) whose deficiency causes a congenital disorder of glycosylation (CDG)-
Ib (MPI-CDG). Mannose supplements improve patients' symptoms because exogenous
mannose can also directly contribute to N-glycan synthesis through Man-6-P. However, the
quantitative contributions of these and other potential pathways to glycosylation are still
unknown. We developed a sensitive GC-MS-based method using [1, 2-13 C] glucose and [4 …
Fru-6-P↔ Man-6-P) whose deficiency causes a congenital disorder of glycosylation (CDG)-
Ib (MPI-CDG). Mannose supplements improve patients' symptoms because exogenous
mannose can also directly contribute to N-glycan synthesis through Man-6-P. However, the
quantitative contributions of these and other potential pathways to glycosylation are still
unknown. We developed a sensitive GC-MS-based method using [1, 2-13 C] glucose and [4 …
