[HTML][HTML] Domain structure of human nuclear DNA helicase II (RNA helicase A)
S Zhang, F Grosse - Journal of Biological Chemistry, 1997 - ASBMB
Full-length human nuclear DNA helicase II (NDH II) was cloned and overexpressed in a
baculovirus-derived expression system. Recombinant NDH II unwound both DNA and RNA.
Limited tryptic digestion produced active helicases with molecular masses of 130 and 100
kDa. The 130-kDa helicase missed a glycine-rich domain (RGG-box) at the carboxyl
terminus, while the 100-kDa form missed both its double-stranded RNA binding domains
(dsRBDs) at the amino terminus and its RGG-box. Hence, the dsRBDs and the RGG-box …
baculovirus-derived expression system. Recombinant NDH II unwound both DNA and RNA.
Limited tryptic digestion produced active helicases with molecular masses of 130 and 100
kDa. The 130-kDa helicase missed a glycine-rich domain (RGG-box) at the carboxyl
terminus, while the 100-kDa form missed both its double-stranded RNA binding domains
(dsRBDs) at the amino terminus and its RGG-box. Hence, the dsRBDs and the RGG-box …