[HTML][HTML] Trehalose dimycolate interferes with FcγR-mediated phagosome maturation through Mincle, SHP-1 and FcγRIIB signalling
EC Patin, AC Geffken, S Willcocks, C Leschczyk… - PloS one, 2017 - journals.plos.org
EC Patin, AC Geffken, S Willcocks, C Leschczyk, A Haas, F Nimmerjahn, R Lang, TH Ward…
PloS one, 2017•journals.plos.orgThe causative agent of tuberculosis, Mycobacterium tuberculosis (M. tuberculosis), contains
an abundant cell wall glycolipid and a crucial virulence factor, trehalose-6, 6'-dimycolate
(TDM). TDM causes delay of phagosome maturation and thus promotes survival of
mycobacteria inside host macrophages by a not fully understood mechanism. TDM signals
through the Monocyte-INducible C-type LEctin (Mincle), a recently identified pattern
recognition receptor. Here we show that recruitment of Mincle by TDM coupled to …
an abundant cell wall glycolipid and a crucial virulence factor, trehalose-6, 6'-dimycolate
(TDM). TDM causes delay of phagosome maturation and thus promotes survival of
mycobacteria inside host macrophages by a not fully understood mechanism. TDM signals
through the Monocyte-INducible C-type LEctin (Mincle), a recently identified pattern
recognition receptor. Here we show that recruitment of Mincle by TDM coupled to …
The causative agent of tuberculosis, Mycobacterium tuberculosis (M. tuberculosis), contains an abundant cell wall glycolipid and a crucial virulence factor, trehalose-6,6’-dimycolate (TDM). TDM causes delay of phagosome maturation and thus promotes survival of mycobacteria inside host macrophages by a not fully understood mechanism. TDM signals through the Monocyte-INducible C-type LEctin (Mincle), a recently identified pattern recognition receptor. Here we show that recruitment of Mincle by TDM coupled to immunoglobulin (Ig)G-opsonised beads during Fcγ receptor (FcγR)-mediated phagocytosis interferes with phagosome maturation. In addition, modulation of phagosome maturation by TDM requires SH2-domain-containing inositol polyphosphate 5’ phosphatase (SHP-1) and the FcγRIIB, which strongly suggests inhibitory downstream signalling of Mincle during phagosome formation. Overall, our study reveals important mechanisms contributing to the virulence of TDM.
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