Different host-cell shutoff strategies related to the matrix protein lead to persistence of vesicular stomatitis virus mutants on fibroblast cells
M Desforges, J Charron, S Bérard, S Beausoleil… - Virus research, 2001 - Elsevier
Acute infection of fibroblastic cell lines by the Indiana strain of vesicular stomatitis virus
(VSV) usually induces dramatic cytopathic effects and shutoff of cellular gene expression.
We have compared a series of independent mutants with differences in shutoff induction and
found that M was mutated either in the N-terminus (M51R) or C-terminus (V221F and
S226R). Furthermore, only double mutants (M mutation and a ts mutation related or not to M)
were able to persist on fibroblast cell lines at 39° C. A more detailed investigation of the …
(VSV) usually induces dramatic cytopathic effects and shutoff of cellular gene expression.
We have compared a series of independent mutants with differences in shutoff induction and
found that M was mutated either in the N-terminus (M51R) or C-terminus (V221F and
S226R). Furthermore, only double mutants (M mutation and a ts mutation related or not to M)
were able to persist on fibroblast cell lines at 39° C. A more detailed investigation of the …