Chloride intracellular channel 1 is an important factor in the lymphatic metastasis of hepatocarcinoma
RK Li, J Zhang, YH Zhang, ML Li, M Wang… - Biomedicine & …, 2012 - Elsevier
RK Li, J Zhang, YH Zhang, ML Li, M Wang, JW Tang
Biomedicine & Pharmacotherapy, 2012•ElsevierWe have previously demonstrated that chloride intracellular channel 1 (CLIC1) is involved in
the lymphatic metastasis of tumors. In this study, a self-designed shRNA sequence of mouse
CLIC1 gene was synthesized and inserted into a pGPU6/GFP/Neo plasmid, then stably
transfected into mouse hepatic carcinoma cell line Hca-F cells to down-regulate the
expression of CLIC1 gene. The levels of expression of CLIC1 mRNA and protein were
detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot …
the lymphatic metastasis of tumors. In this study, a self-designed shRNA sequence of mouse
CLIC1 gene was synthesized and inserted into a pGPU6/GFP/Neo plasmid, then stably
transfected into mouse hepatic carcinoma cell line Hca-F cells to down-regulate the
expression of CLIC1 gene. The levels of expression of CLIC1 mRNA and protein were
detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot …
We have previously demonstrated that chloride intracellular channel 1 (CLIC1) is involved in the lymphatic metastasis of tumors. In this study, a self-designed shRNA sequence of mouse CLIC1 gene was synthesized and inserted into a pGPU6/GFP/Neo plasmid, then stably transfected into mouse hepatic carcinoma cell line Hca-F cells to down-regulate the expression of CLIC1 gene. The levels of expression of CLIC1 mRNA and protein were detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot (WB) analysis, respectively. The down-regulation of CLIC1 enhanced proliferative activity, increased the ratio of G2/M and decreased percentage of apoptosis. In addition, the capability of migration and invasion decreased significantly. The results indicate that CLIC1 is a critical factor in the development of lymphatic metastasis.
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