Evidence for a functional role of Shc proteins in mitogenic signaling induced by insulin, insulin-like growth factor-1, and epidermal growth factor.

T Sasaoka, DW Rose, BH Jhun, AR Saltiel… - Journal of Biological …, 1994 - ASBMB
Shc proteins contain a single SH2 domain, lack catalytic activity, and are substrates for
activated receptors for insulin, insulin-like growth factor-1 (IGF-1), and epidermal growth
factor (EGF). Treatment with these growth factors induced rapid tyrosine phosphorylation of
Shc. We investigated the potential role of Shc in mitogenic signaling. Affinity-purified
antibodies were microinjected into living Rat1 fibroblasts overexpressing human insulin
receptors. Bromodeoxyuridine incorporation into newly synthesized DNA was subsequently …