[HTML][HTML] Two Naturally Occurring Mutant Insulin Receptors Phosphorylate Insulin Receptor Substrate-1 (IRS-1) but Fail to Mediate the Biological Effects of Insulin …
A Krook, DE Moller, K Dib, S O'Rahilly - Journal of Biological Chemistry, 1996 - ASBMB
Two naturally occurring mutant insulin receptors, Arg-1174→ Gln and Leu-1178→ Pro,
found in patients with dominantly inherited Type A insulin resistance, showed unusual
signaling properties when stably expressed in Chinese hamster ovary (CHO) cells. Both
mutant receptors were expressed on the cell surface and bound insulin normally, but
showed markedly impaired autophosphorylation in response to insulin. In addition, the in
vitro tyrosine kinase activity of both mutant receptors toward an artificial substrate was also …
found in patients with dominantly inherited Type A insulin resistance, showed unusual
signaling properties when stably expressed in Chinese hamster ovary (CHO) cells. Both
mutant receptors were expressed on the cell surface and bound insulin normally, but
showed markedly impaired autophosphorylation in response to insulin. In addition, the in
vitro tyrosine kinase activity of both mutant receptors toward an artificial substrate was also …