[HTML][HTML] The extracellular signal-regulated kinase pathway regulates the phosphorylation of 4E-BP1 at multiple sites
The phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), a potent stimulator of Erk,
leads to the phosphorylation of 4E-BP1 and its dissociation from eIF4E. In contrast to
agonists such as insulin, this occurs independently of PKB activation. In this report, we
investigate the mechanism by which TPA regulates 4E-BP1 phosphorylation. Treatment of
HEK293 cells with TPA was found to result in the phosphorylation of 4E-BP1 at Ser 64, Thr
69, and Thr 36/45. The TPA-stimulated phosphorylation of all these sites is sensitive to …
leads to the phosphorylation of 4E-BP1 and its dissociation from eIF4E. In contrast to
agonists such as insulin, this occurs independently of PKB activation. In this report, we
investigate the mechanism by which TPA regulates 4E-BP1 phosphorylation. Treatment of
HEK293 cells with TPA was found to result in the phosphorylation of 4E-BP1 at Ser 64, Thr
69, and Thr 36/45. The TPA-stimulated phosphorylation of all these sites is sensitive to …