[PDF][PDF] Detection of SARS-CoV-2-specific humoral and cellular immunity in COVID-19 convalescent individuals

L Ni, F Ye, ML Cheng, Y Feng, YQ Deng, H Zhao… - Immunity, 2020 - cell.com
L Ni, F Ye, ML Cheng, Y Feng, YQ Deng, H Zhao, P Wei, J Ge, M Gou, X Li, L Sun, T Cao…
Immunity, 2020cell.com
Summary The World Health Organization has declared SARS-CoV-2 virus outbreak a
worldwide pandemic. However, there is very limited understanding on the immune
responses, especially adaptive immune responses to SARS-CoV-2 infection. Here, we
collected blood from COVID-19 patients who have recently become virus-free, and therefore
were discharged, and detected SARS-CoV-2-specific humoral and cellular immunity in eight
newly discharged patients. Follow-up analysis on another cohort of six patients 2 weeks post …
Summary
The World Health Organization has declared SARS-CoV-2 virus outbreak a worldwide pandemic. However, there is very limited understanding on the immune responses, especially adaptive immune responses to SARS-CoV-2 infection. Here, we collected blood from COVID-19 patients who have recently become virus-free, and therefore were discharged, and detected SARS-CoV-2-specific humoral and cellular immunity in eight newly discharged patients. Follow-up analysis on another cohort of six patients 2 weeks post discharge also revealed high titers of immunoglobulin G (IgG) antibodies. In all 14 patients tested, 13 displayed serum-neutralizing activities in a pseudotype entry assay. Notably, there was a strong correlation between neutralization antibody titers and the numbers of virus-specific T cells. Our work provides a basis for further analysis of protective immunity to SARS-CoV-2, and understanding the pathogenesis of COVID-19, especially in the severe cases. It also has implications in developing an effective vaccine to SARS-CoV-2 infection.
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