[13] Isolation of pancreatic islets and primary culture of the intact microorgans or of dispersed islet cells
CB Wollheim, P Meda, PA Halban - Methods in enzymology, 1990 - Elsevier
Publisher Summary This chapter discusses the isolation of pancreatic islets and primary
culture of intact micro organ or of dispersed islet cells. This chapter discusses the anatomical
distribution and cellular organization of pancreatic islets. This chapter describes the
microdissection for isolation methods for obtaining islets from the adult pancreas. The main
advantage of microdissection is to avoid the use of enzymes that may damage islet cells or
alter their functioning. Thus, differences between microdissected and collagenase-isolated …
culture of intact micro organ or of dispersed islet cells. This chapter discusses the anatomical
distribution and cellular organization of pancreatic islets. This chapter describes the
microdissection for isolation methods for obtaining islets from the adult pancreas. The main
advantage of microdissection is to avoid the use of enzymes that may damage islet cells or
alter their functioning. Thus, differences between microdissected and collagenase-isolated …