Microfluidic LIPS for serum antibody detection: demonstration of a rapid test for HSV-2 infection
Biomedical microdevices, 2011•Springer
There is great interest in point-of-care antibody testing for the diagnosis of infectious and
autoimmune diseases. As a first step in the development of self-contained and miniaturized
devices for highly quantitative antibody detection, we demonstrate the application of
Luciferase Immunoprecipitation Systems (LIPS) technology in a microfluidic format. Protein
A/G was immobilized on the walls of PDMS-glass microchannels of 500 nL volume. The
assay proceeds with the simultaneous introduction of plasma and Renilla luciferase–tagged …
autoimmune diseases. As a first step in the development of self-contained and miniaturized
devices for highly quantitative antibody detection, we demonstrate the application of
Luciferase Immunoprecipitation Systems (LIPS) technology in a microfluidic format. Protein
A/G was immobilized on the walls of PDMS-glass microchannels of 500 nL volume. The
assay proceeds with the simultaneous introduction of plasma and Renilla luciferase–tagged …
Abstract
There is great interest in point-of-care antibody testing for the diagnosis of infectious and autoimmune diseases. As a first step in the development of self-contained and miniaturized devices for highly quantitative antibody detection, we demonstrate the application of Luciferase Immunoprecipitation Systems (LIPS) technology in a microfluidic format. Protein A/G was immobilized on the walls of PDMS-glass microchannels of 500 nL volume. The assay proceeds with the simultaneous introduction of plasma and Renilla luciferase–tagged antigens. Following washing, coelenterazine substrate was added and bound antigen-luciferase measured by chemiluminescence. Total assay time, including rinsing and detection, is under 10 min. Using these stable microfluidic devices, high diagnostic performance (100% sensitivity and 100% specificity) was achieved for the diagnosis of HSV-2 infection. Based on these findings, the LIPS microfluidic format should readily lend itself to automation and the transfer to portable instrumentation.
Springer