Ophthalmic acid is a marker of oxidative stress in plants as in animals
L Servillo, D Castaldo, A Giovane, R Casale… - … et Biophysica Acta (BBA …, 2018 - Elsevier
Biochimica et Biophysica Acta (BBA)-General Subjects, 2018•Elsevier
Background Ophthalmic acid (OPH), γ-glutamyl-L-2-aminobutyryl-glycine, a tripeptide
analogue of glutathione (GSH), has recently captured considerable attention as a biomarker
of oxidative stress in animals. The OPH and GSH biosynthesis, as well as some biochemical
behaviors, are very similar. Here, we sought to investigate the presence of OPH in plants
and its possible relationship with GSH, known to possess multiple functions in the plant
development, growth and response to environmental changes. Methods HPLC-ESI-MS/MS …
analogue of glutathione (GSH), has recently captured considerable attention as a biomarker
of oxidative stress in animals. The OPH and GSH biosynthesis, as well as some biochemical
behaviors, are very similar. Here, we sought to investigate the presence of OPH in plants
and its possible relationship with GSH, known to possess multiple functions in the plant
development, growth and response to environmental changes. Methods HPLC-ESI-MS/MS …
Background
Ophthalmic acid (OPH), γ-glutamyl-L-2-aminobutyryl-glycine, a tripeptide analogue of glutathione (GSH), has recently captured considerable attention as a biomarker of oxidative stress in animals. The OPH and GSH biosynthesis, as well as some biochemical behaviors, are very similar. Here, we sought to investigate the presence of OPH in plants and its possible relationship with GSH, known to possess multiple functions in the plant development, growth and response to environmental changes.
Methods
HPLC-ESI-MS/MS analysis was used to examine the occurrence of OPH in leaves from various plant species, and flours from several plant seeds. Different types of oxidative stress, i.e., water, dark, paraquat, and cadmium stress, were induced in rye, barley, oat, and winter wheat leaves to evaluate the effects on the levels of OPH and its metabolic precursors.
Results
OPH and its dipeptide precursor, γ-glutamyl-2-aminobutyric acid, were found to occur in phylogenetically distant plants. Interestingly, the levels of OPH were tightly associated with the oxidative stress tested. Levels of OPH precursors, γ-glutamyl-2-aminobutyric acid and 2-aminobutyric acid, the latter efficiently formed in plants via biosynthetic pathways absent in the animal kingdom, were also found to increase during oxidative stress.
Conclusions
OPH occurs in plants and its levels are tightly associated with oxidative stress.
General significance
OPH behaves as an oxidative stress marker and its biogenesis might occur through a biochemical pathway common to many living organisms.
Elsevier