Purification of hemoglobin from red blood cells using tangential flow filtration and immobilized metal ion affinity chromatography
Two methods for purifying hemoglobin (Hb) from red blood cells (RBCs) are compared. In
the first method, red blood cell lysate is clarified with a 50nm tangential flow filter and
hemoglobin is purified using immobilized metal ion affinity chromatography (IMAC). In the
second method, RBC lysate is processed with 50nm, 500kDa, and 50–100kDa tangential
flow filters, then hemoglobin is purified with IMAC. Our results show that the hemoglobins
from both processes produce identical Hb products that are ultrapure and retain their …
the first method, red blood cell lysate is clarified with a 50nm tangential flow filter and
hemoglobin is purified using immobilized metal ion affinity chromatography (IMAC). In the
second method, RBC lysate is processed with 50nm, 500kDa, and 50–100kDa tangential
flow filters, then hemoglobin is purified with IMAC. Our results show that the hemoglobins
from both processes produce identical Hb products that are ultrapure and retain their …