A single oral sensitization to peanut without adjuvant leads to anaphylaxis in mice

B Proust, C Astier, S Jacquenet, V Ogier… - International archives of …, 2008 - karger.com
B Proust, C Astier, S Jacquenet, V Ogier, E Magueur, O Roitel, C Belcourt, M Morisset…
International archives of allergy and immunology, 2008karger.com
Background: A model of peanut food allergy has been developed in mice using a simple
sensitization protocol leading to a quantitatively measurable allergic response. Methods:
C3H/HeJ mice received a single intragastric administration of whole peanut (80 mg) without
adjuvant. Two weeks later, intraperitoneal challenge with peanut extract led to a severe
anaphylaxis. Results: Anaphylactic reaction was evidenced by vascular leakage, severe
clinical symptoms, a drop in body temperature, a decrease in breathing rate and also by …
Background
A model of peanut food allergy has been developed in mice using a simple sensitization protocol leading to a quantitatively measurable allergic response.
Methods
C3H/HeJ mice received a single intragastric administration of whole peanut (80 mg) without adjuvant. Two weeks later, intraperitoneal challenge with peanut extract led to a severe anaphylaxis.
Results
Anaphylactic reaction was evidenced by vascular leakage, severe clinical symptoms, a drop in body temperature, a decrease in breathing rate and also by increased concentrations of serum mouse mast cell protease-1. Sensitization to peanut was demonstrated by positive skin tests (ear swelling test and intradermal skin testing) and increased peanut-specific IgE levels.
Conclusions
Thus, we obtained a model of severe peanut hypersensitivity within 2 weeks following single oral exposure without adjuvant. This model may be useful for further basic and applied studies on peanut allergy.
Karger