Antigenic cross-reactivity between severe acute respiratory syndrome—associated coronavirus and human coronaviruses 229E and OC43
X Che, L Qiu, Z Liao, Y Wang, K Wen… - Journal of Infectious …, 2005 - academic.oup.com
X Che, L Qiu, Z Liao, Y Wang, K Wen, Y Pan, W Hao, Y Mei, VCC Cheng, K Yuen
Journal of Infectious Diseases, 2005•academic.oup.comCross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been
systematically studied. By use of Western blot analysis, indirect immunofluorescence assay
(IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between
severe acute respiratory syndrome (SARS)—associated coronavirus (SARS-CoV) and 2
HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5
of 11 and 10 of 11 patients with SARS, paired serum samples showed a⩾ 4-fold increase in …
systematically studied. By use of Western blot analysis, indirect immunofluorescence assay
(IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between
severe acute respiratory syndrome (SARS)—associated coronavirus (SARS-CoV) and 2
HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5
of 11 and 10 of 11 patients with SARS, paired serum samples showed a⩾ 4-fold increase in …
Abstract
Cross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been systematically studied. By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndrome (SARS)—associated coronavirus (SARS-CoV) and 2 HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5 of 11 and 10 of 11 patients with SARS, paired serum samples showed a ⩾4-fold increase in antibody titers against HCoV-229E and HCoV-OC43, respectively, by IFA. Overall, serum samples from convalescent patients who had SARS had a 1-way cross-reactivity with the 2 known HCoVs. Antigens of SARS-CoV and HCoV-OC43 were more cross-reactive than were those of SARS-CoV and HCoV-229E.
Oxford University Press