Generation of B lymphoma cell lines from knockout mice by transformation in vivo with an Eμ-myc transgene
LM Corcoran, S Tawfilis, LJ Barlow - Journal of immunological methods, 1999 - Elsevier
While a great deal has been learned about the genetic control of B lymphocyte behavior
through the observation of primary B cells from mice bearing targeted gene mutations, such
studies can be restricted by the limited number and longevity of the cells ex vivo, by their
heterogeneity, and by the inability to apply further genetic manipulations. Here we describe
a protocol for the efficient derivation of mutant B lymphoma cell lines, by crossing mice
bearing targeted mutations in genes affecting mature B cell function with transgenic mice …
through the observation of primary B cells from mice bearing targeted gene mutations, such
studies can be restricted by the limited number and longevity of the cells ex vivo, by their
heterogeneity, and by the inability to apply further genetic manipulations. Here we describe
a protocol for the efficient derivation of mutant B lymphoma cell lines, by crossing mice
bearing targeted mutations in genes affecting mature B cell function with transgenic mice …