[HTML][HTML] Ablation of the ASCT2 (SLC1A5) gene encoding a neutral amino acid transporter reveals transporter plasticity and redundancy in cancer cells
A Bröer, G Gauthier-Coles, F Rahimi… - Journal of Biological …, 2019 - ASBMB
The neutral amino acid transporter solute carrier family 1 member 5 (SLC1A5 or ASCT2) is
overexpressed in many cancers. To identify its roles in tumors, we employed 143B
osteosarcoma cells and HCC1806 triple-negative breast cancer cells with or without ASCT2
deletion. ASCT2ko 143B cells grew well in standard culture media, but ASCT2 was required
for optimal growth at< 0.5 mm glutamine, with tumor spheroid growth and monolayer
migration of 143B ASCT2ko cells being strongly impaired at lower glutamine concentrations …
overexpressed in many cancers. To identify its roles in tumors, we employed 143B
osteosarcoma cells and HCC1806 triple-negative breast cancer cells with or without ASCT2
deletion. ASCT2ko 143B cells grew well in standard culture media, but ASCT2 was required
for optimal growth at< 0.5 mm glutamine, with tumor spheroid growth and monolayer
migration of 143B ASCT2ko cells being strongly impaired at lower glutamine concentrations …