Ferroportin 1 (FPN1) is transmembrane protein involved in iron homeostasis. In the duodenum, FPN1 localizes to the basolateral surface of enterocytes where it appears to export iron out of the cell and into the portal circulation. FPN1 is also abundantly expressed in reticuloendothelial macrophages of the liver, spleen, and bone marrow, suggesting that this protein serves as an iron exporter in cells that recycle iron from senescent red blood cells. To directly test the hypothesis that FPN1 functions in the export of iron after erythrophagocytosis, FPN1 was stably expressed in J774 mouse macrophages by using retroviral transduction, and release of ⁵⁹Fe after phagocytosis of ⁵⁹Fe-labeled rat erythrocytes was measured. J774 cells overexpressing FPN1 released 70% more ⁵⁹Fe after erythrophagocytosis than control cells, consistent with a role in the recycling of iron from senescent red cells. Treatment of cells with the peptide hormone hepcidin, a systemic regulator of iron metabolism, dramatically decreased FPN1 protein levels and significantly reduced the efflux of ⁵⁹Fe after erythrophagocytosis. Subsequent fractionation of the total released ⁵⁹Fe into heme and nonheme compounds revealed that hepcidin treatment reduced the release of nonheme ⁵⁹Fe by 50% and 25% from control and FPN1-overexpressing cells, respectively, but did not diminish efflux of ⁵⁹Fe-heme. We conclude that FPN1 is directly involved in the export of iron during erythrocyte-iron recycling by macrophages.