Transmission electron microscopy (TEM) is extremely useful for visualizing microglial,
oligodendrocytic, astrocytic, and neuronal subcellular compartments (dendrite, dendritic
spine, axon, axon terminal, perikaryon), as well as their intracellular organelles and
cytoskeleton, in the central nervous system at high spatial resolution. Combined with TEM,
pre-embedding immunocytochemistry allows the discrimination of cellular elements with few
distinctive features and identification criteria (eg, microglial perikarya and processes, when …