The pathological aggregation of the tau protein is a common characteristic of many
neurodegenerative diseases. There is strong interest in characterizing the potentially toxic
nature of tau oligomers. These nonfibrillar, soluble multimers appear to be more toxic than
neurofibrillary tangles made up of filamentous tau. However, reliable production, purification,
and verification of tau oligomers can provide certain challenges. Here, we provide a series
of methods that address these issues. First, recombinant tau is produced using Escherichia …