Isolation of cardiomyocyte nuclei from post-mortem tissue
O Bergmann, S Jovinge - JoVE (Journal of Visualized Experiments), 2012 - jove.com
Identification of cardiomyocyte nuclei has been challenging in tissue sections as most
strategies rely only on cytoplasmic marker proteins1. Rare events in cardiac myocytes such
as proliferation and apoptosis require an accurate identification of cardiac myocyte nuclei to
analyze cellular renewal in homeostasis and in pathological conditions2. Here, we provide a
method to isolate cardiomyocyte nuclei from post mortem tissue by density sedimentation
and immunolabeling with antibodies against pericentriolar material 1 (PCM-1) and …
strategies rely only on cytoplasmic marker proteins1. Rare events in cardiac myocytes such
as proliferation and apoptosis require an accurate identification of cardiac myocyte nuclei to
analyze cellular renewal in homeostasis and in pathological conditions2. Here, we provide a
method to isolate cardiomyocyte nuclei from post mortem tissue by density sedimentation
and immunolabeling with antibodies against pericentriolar material 1 (PCM-1) and …