Co-Transplantation of Fetal Bone Tissue Facilitates the Development and Reconstitution in Human B Cells in Humanized NOD/SCID/IL-2Rγnull (NSG) Mice
M Kim, B Choi, SY Kim, JH Yang, CR Roh… - Journal of clinical …, 2011 - Springer
M Kim, B Choi, SY Kim, JH Yang, CR Roh, KY Lee, SJ Kim
Journal of clinical immunology, 2011•SpringerBackground In terms of the function and reconstitution efficacy of human immune cells, co-
transplantation of human fetal tissues, such as thymus and liver, with CD34+ hematopoietic
stem cells (HSCs) has potential advantages in the generation of humanized mice. Objective
and Methods To examine the effects of bone tissues in the reconstitution of human immune
cells, particularly in B cells, we generated a new humanized mice co-transplanted with
human fetal thymus (hFT)/fetal bone (hFB) tissues and human fetal liver-derived CD34+ …
transplantation of human fetal tissues, such as thymus and liver, with CD34+ hematopoietic
stem cells (HSCs) has potential advantages in the generation of humanized mice. Objective
and Methods To examine the effects of bone tissues in the reconstitution of human immune
cells, particularly in B cells, we generated a new humanized mice co-transplanted with
human fetal thymus (hFT)/fetal bone (hFB) tissues and human fetal liver-derived CD34+ …
Background
In terms of the function and reconstitution efficacy of human immune cells, co-transplantation of human fetal tissues, such as thymus and liver, with CD34+ hematopoietic stem cells (HSCs) has potential advantages in the generation of humanized mice.
Objective and Methods
To examine the effects of bone tissues in the reconstitution of human immune cells, particularly in B cells, we generated a new humanized mice co-transplanted with human fetal thymus (hFT)/fetal bone (hFB) tissues and human fetal liver-derived CD34+ cells.
Results
Humanized mice exhibited effective reconstitution of human immune cells earlier compared to control humanized mice. In terms of quantity, the number of immune cells, such as human T, B, and monocyte/macrophages was significantly increased. Furthermore, significant increase of B cell progenitors and immature/naïve B cells could be detected in the bone marrow and spleen of humanized mice.
Conclusion
Our results demonstrate that co-transplantation of hFB tissue may facilitate the reconstitution of human B and T cells, and therefore the humanized model may be used to develop therapeutic human antibodies for clinical use.
Springer