[HTML][HTML] Rapid Accumulation of CD14+CD11c+ Dendritic Cells in Gut Mucosa of Celiac Disease after in vivo Gluten Challenge
ACR Beitnes, M Raki, M Brottveit, KEA Lundin… - PloS one, 2012 - journals.plos.org
PloS one, 2012•journals.plos.org
Background Of antigen-presenting cells (APCs) expressing HLA-DQ molecules in the celiac
disease (CD) lesion, CD11c+ dendritic cells (DCs) co-expressing the monocyte marker
CD14 are increased, whereas other DC subsets (CD1c+ or CD103+) and CD163+ CD11c−
macrophages are all decreased. It is unclear whether these changes result from chronic
inflammation or whether they represent early events in the gluten response. We have
addressed this in a model of in vivo gluten challenge. Methods Treated HLA-DQ2+ CD …
disease (CD) lesion, CD11c+ dendritic cells (DCs) co-expressing the monocyte marker
CD14 are increased, whereas other DC subsets (CD1c+ or CD103+) and CD163+ CD11c−
macrophages are all decreased. It is unclear whether these changes result from chronic
inflammation or whether they represent early events in the gluten response. We have
addressed this in a model of in vivo gluten challenge. Methods Treated HLA-DQ2+ CD …
Background
Of antigen-presenting cells (APCs) expressing HLA-DQ molecules in the celiac disease (CD) lesion, CD11c+ dendritic cells (DCs) co-expressing the monocyte marker CD14 are increased, whereas other DC subsets (CD1c+ or CD103+) and CD163+CD11c− macrophages are all decreased. It is unclear whether these changes result from chronic inflammation or whether they represent early events in the gluten response. We have addressed this in a model of in vivo gluten challenge.
Methods
Treated HLA-DQ2+ CD patients (n = 12) and HLA-DQ2+ gluten-sensitive control subjects (n = 12) on a gluten-free diet (GFD) were orally challenged with gluten for three days. Duodenal biopsies obtained before and after gluten challenge were subjected to immunohistochemistry. Single cell digests of duodenal biopsies from healthy controls (n = 4), treated CD (n = 3) and untreated CD (n = 3) patients were analyzed by flow cytometry.
Results
In treated CD patients, the gluten challenge increased the density of CD14+CD11c+ DCs, whereas the density of CD103+CD11c+ DCs and CD163+CD11c− macrophages decreased, and the density of CD1c+CD11c+ DCs remained unchanged. Most CD14+CD11c+ DCs co-expressed CCR2. The density of neutrophils also increased in the challenged mucosa, but in most patients no architectural changes or increase of CD3+ intraepithelial lymphocytes (IELs) were found. In control tissue no significant changes were observed.
Conclusions
Rapid accumulation of CD14+CD11c+ DCs is specific to CD and precedes changes in mucosal architecture, indicating that this DC subset may be directly involved in the immunopathology of the disease. The expression of CCR2 and CD14 on the accumulating CD11c+ DCs indicates that these cells are newly recruited monocytes.
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