Endothelial dysfunction is associated with early development of cardiovascular disease,
making longitudinal measurements desirable. We devised a protocol using laser Doppler
imaging (LDI) and iontophoresis of acetylcholine (ACh) and sodium nitroprusside (SNP) to
assess the skin microcirculation longitudinally in mice every 4weeks for 24weeks in two
groups of C57BL/6 mice, chow versus high-cholesterol diet (known to induce endothelial
dysfunction). LDI measurements were compared with vascular function (isometric tension) …