Irradiated mesenchymal stem cells improve the ex vivo expansion of hematopoietic progenitors by partly mimicking the bone marrow endosteal environment
Abstract Mesenchymal Stem Cells (MSCs) regulate the growth and differentiation of
Hematopoietic Progenitor cells (HPCs) through the release of soluble factors or through their
differentiation into osteoblasts. We recently demonstrated that expansion of megakaryocyte
(MK) progenitors ex vivo had reached a plateau when CD34+ cells were grown with two
optimized cytokine cocktails developed for the growth of MK. Hence, we sought to determine
whether co-culture of CD34+ cells with Bone Marrow (BM) MSCs could further increase the …
Hematopoietic Progenitor cells (HPCs) through the release of soluble factors or through their
differentiation into osteoblasts. We recently demonstrated that expansion of megakaryocyte
(MK) progenitors ex vivo had reached a plateau when CD34+ cells were grown with two
optimized cytokine cocktails developed for the growth of MK. Hence, we sought to determine
whether co-culture of CD34+ cells with Bone Marrow (BM) MSCs could further increase the …