4-1BB-based Isolation and Expansion of CD8+ T Cells Specific for Self-Tumor and Non–Self-Tumor Antigens for Adoptive T-cell Therapy
Adoptive T-cell therapy is a promising approach to the immunotherapy of cancer, but for it to
be a general cancer therapy a simple and standardized procedure for producing tumor-
specific CD8+ T cells is needed. On the basis of a unique property of 4-1BB (CD137), the
selective expression on activated T cells, we have developed a simple and practical protocol
to produce antigen-specific CD8+ T cells from peripheral blood mononuclear cells. We have
proved the feasibility of this procedure by isolating and expanding cytomegalovirus-specific …
be a general cancer therapy a simple and standardized procedure for producing tumor-
specific CD8+ T cells is needed. On the basis of a unique property of 4-1BB (CD137), the
selective expression on activated T cells, we have developed a simple and practical protocol
to produce antigen-specific CD8+ T cells from peripheral blood mononuclear cells. We have
proved the feasibility of this procedure by isolating and expanding cytomegalovirus-specific …