[HTML][HTML] Tumour-suppressive microRNA-29s directly regulate LOXL2 expression and inhibit cancer cell migration and invasion in renal cell carcinoma
R Nishikawa, T Chiyomaru, H Enokida, S Inoguchi… - FEBS letters, 2015 - Elsevier
R Nishikawa, T Chiyomaru, H Enokida, S Inoguchi, T Ishihara, R Matsushita, Y Goto…
FEBS letters, 2015•ElsevierHere, we found that members of the microRNA-29 family (miR-29a/b/c;“miR-29s”) were
significantly reduced in clear cell renal cell carcinoma (ccRCC) tissues, suggesting that they
functioned as tumour suppressors. Restoration of all mature members of the miR-29 family
inhibited cancer cell proliferation, migration and invasion. LOXL2 was a direct target gene of
miR-29s, as shown by genome-wide gene expression analysis and luciferase reporter
assay. Overexpressed LOXL2 was confirmed in ccRCC clinical specimens, and silencing of …
significantly reduced in clear cell renal cell carcinoma (ccRCC) tissues, suggesting that they
functioned as tumour suppressors. Restoration of all mature members of the miR-29 family
inhibited cancer cell proliferation, migration and invasion. LOXL2 was a direct target gene of
miR-29s, as shown by genome-wide gene expression analysis and luciferase reporter
assay. Overexpressed LOXL2 was confirmed in ccRCC clinical specimens, and silencing of …
Abstract
Here, we found that members of the microRNA-29 family (miR-29a/b/c; “miR-29s”) were significantly reduced in clear cell renal cell carcinoma (ccRCC) tissues, suggesting that they functioned as tumour suppressors. Restoration of all mature members of the miR-29 family inhibited cancer cell proliferation, migration and invasion. LOXL2 was a direct target gene of miR-29s, as shown by genome-wide gene expression analysis and luciferase reporter assay. Overexpressed LOXL2 was confirmed in ccRCC clinical specimens, and silencing of LOXL2 inhibited cancer cell migration and invasion in ccRCC cell lines. Our data demonstrated that the miR-29s-LOXL2 axis contributed to cancer cell migration and invasion in ccRCC.
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