Understanding the mechanisms of replication stress response following genotoxic stress
induction is rapidly emerging as a central theme in cell survival and human disease. The
DNA fiber assay is one of the most powerful tools to study alterations in replication fork
dynamics genome-wide at single-molecule resolution. This approach relies on the ability of
many organisms to incorporate thymidine analogs into replicating DNA and is widely used to
study how genotoxic agents perturb DNA replication. Here, we review different approaches …