An in-vitro preparation of isolated enteric neurons and glia from the myenteric plexus of the adult mouse
TH Smith, J Ngwainmbi, JR Grider, WL Dewey… - JoVE (Journal of …, 2013 - jove.com
The enteric nervous system is a vast network of neurons and glia running the length of the
gastrointestinal tract that functionally controls gastrointestinal motility. A procedure for the
isolation and culture of a mixed population of neurons and glia from the myenteric plexus is
described. The primary cultures can be maintained for over 7 days, with connections
developing among the neurons and glia. The longitudinal muscle strip with the attached
myenteric plexus is stripped from the underlying circular muscle of the mouse ileum or colon …
gastrointestinal tract that functionally controls gastrointestinal motility. A procedure for the
isolation and culture of a mixed population of neurons and glia from the myenteric plexus is
described. The primary cultures can be maintained for over 7 days, with connections
developing among the neurons and glia. The longitudinal muscle strip with the attached
myenteric plexus is stripped from the underlying circular muscle of the mouse ileum or colon …