Unfolded protein response (UPR) is a key cellular defense mechanism associated with
many human “conformational” diseases, including heart diseases, neurodegeneration, and
metabolic syndrome. One of the major obstacles that have hindered our further
understanding of physiological UPR and its future therapeutic potential is our inability to
detect and quantitate ER stress and UPR activation under physiological and pathological
conditions, where ER stress is perceivably very mild. Here, we describe a Phos-tag-based …