[HTML][HTML] Comparative proteomic phenotyping of cell lines and primary cells to assess preservation of cell type-specific functions
Biological experiments are most often performed with immortalized cell lines because they
are readily available and can be expanded without limitation. However, cell lines may differ
from the in vivo situation in important aspects. Here we introduce a straightforward
methodology to compare cell lines to their cognate primary cells and to derive a comparative
functional phenotype. We used SILAC (stable isotope labeling by amino acids in cell culture)
for quantitative, mass spectrometry-based comparison of the hepatoma cell line Hepa1–6 …
are readily available and can be expanded without limitation. However, cell lines may differ
from the in vivo situation in important aspects. Here we introduce a straightforward
methodology to compare cell lines to their cognate primary cells and to derive a comparative
functional phenotype. We used SILAC (stable isotope labeling by amino acids in cell culture)
for quantitative, mass spectrometry-based comparison of the hepatoma cell line Hepa1–6 …