Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels
N Dyballa, S Metzger - JoVE (Journal of Visualized Experiments), 2009 - jove.com
Coomassie Brilliant Blue (CBB) is a dye commonly used for the visualization of proteins
separated by SDS-PAGE, offering a simple staining procedure and high quantitation.
Furthermore, it is completely compatible with mass spectrometric protein identification. But
despite these advantages, CBB is regarded to be less sensitive than silver or fluorescence
stainings and therefore rarely used for the detection of proteins in analytical gel-based
proteomic approaches. Several improvements of the original Coomassie protocol1 have …
separated by SDS-PAGE, offering a simple staining procedure and high quantitation.
Furthermore, it is completely compatible with mass spectrometric protein identification. But
despite these advantages, CBB is regarded to be less sensitive than silver or fluorescence
stainings and therefore rarely used for the detection of proteins in analytical gel-based
proteomic approaches. Several improvements of the original Coomassie protocol1 have …