Importance of murine Vδ1+γδ T cells expressing interferon‐γ and interleukin‐17A in innate protection against Listeria monocytogenes infection

S Hamada, M Umemura, T Shiono, H Hara… - …, 2008 - Wiley Online Library
S Hamada, M Umemura, T Shiono, H Hara, K Kishihara, K Tanaka, H Mayuzumi, T Ohta…
Immunology, 2008Wiley Online Library
Murine γδ T cells participate in the innate immune response against infection by an
intracellular pathogen Listeria monocytogenes. Vδ1+ γδ T cells coexpressing Vγ6 are a
major γδ T‐cell subpopulation induced at an early stage of L. monocytogenes infection in
the livers of infected mice. To investigate the protective role of the Vγ6/Vδ1+ γδ T cells
against L. monocytogenes infection, Vδ1 gene‐deficient (Vδ1−/−) mice were analysed
because these mice selectively lacked a Vγ6/Vδ1+ γδ T‐cell subpopulation in the L …
Summary
Murine γδ T cells participate in the innate immune response against infection by an intracellular pathogen Listeria monocytogenes. Vδ1+γδ T cells coexpressing Vγ6 are a major γδ T‐cell subpopulation induced at an early stage of L. monocytogenes infection in the livers of infected mice. To investigate the protective role of the Vγ6/Vδ1+γδ T cells against L. monocytogenes infection, Vδ1 gene‐deficient (Vδ1−/−) mice were analysed because these mice selectively lacked a Vγ6/Vδ1+γδ T‐cell subpopulation in the L. monocytogenes‐infected liver. The Vδ1−/− mice showed increased bacterial burden in the liver and spleen, and decreased survival rate at an early stage of L. monocytogenes infection when compared to wild‐type mice. Histological examination showed abscess‐like lesions and unorganized distribution of macrophages in the liver of the Vδ1−/− mice but not in the wild‐type mice after L. monocytogenes infection. The Vγ6/Vδ1+γδ T cells produced interferon‐γ and interleukin‐17A. All the results suggest that murine Vγ6/Vδ1+γδ T cells control the innate protective response against L. monocytogenes infection through production of the proinflammatory cytokines interferon‐γ and interleukin‐17A in the infected liver.
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